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R&D Systems ltb4 parameter assay r d systems
Ltb4 Parameter Assay R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ltb4+parameter+assay+r+d+systems/pm39096902-923-168-171?v=R%26D+Systems
Average 94 stars, based on 66 article reviews

ltb4 parameter assay r d systems - by Bioz Stars, 2026-08

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Figure 4. PMN Vascular Clustering during Candidemia Is Dependent on LTB4 In Vivo (A) PMN clustering (white) is compared among C57BL/6, C3/, and anti-C5aR mAb-treated mice following 1 hr of i.v. live C. albicans. Scale bar, 200 mm. (B and C) (B) Mean cluster area and (C) max skeletal length of clusters were compared. One-way ANOVA, Holm-Sidak’s multiple comparison. (D and E) Lung LTB4 levels of C57BL/6 and C3/ mice (D), two-way ANOVA, Holm-Sidak’s multiple comparison, and following anti-C5aR mAb-treated mice (E). (F) LTB4 in isolated pulmonary PMN with or without C. albicans infection (1 hr). Unpaired two-tailed t test. (G) A representative image of PMN clusters (white) in LTB4R/ mice following C. albicans (1 hr). Scale bar, 200 mm. (H and I) (H) Mean cluster area and (I) max. skeletal length of PMN clusters after C. albicans (1 hr). (J–L) 1 3 107 A488 zymosan or 1 3 107 A488 E. coli bioparticles (green) were injected i.v. during lung imaging. (J) Intravital images are shown at 60 min. Scale bar, 100 mm. Clusters were quantiﬁed as (K) max skeletal length and (L) mean cluster area. ANOVA was performed for panels with more than two groups, while unpaired two-tailed t test were used to compare two groups. n = at least 3 for each panel. Error bars represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

Journal: Cell host & microbe

Article Title: Leukotriene B4-Mediated Neutrophil Recruitment Causes Pulmonary Capillaritis during Lethal Fungal Sepsis.

doi: 10.1016/j.chom.2017.11.009

Figure Lengend Snippet: Figure 4. PMN Vascular Clustering during Candidemia Is Dependent on LTB4 In Vivo (A) PMN clustering (white) is compared among C57BL/6, C3/, and anti-C5aR mAb-treated mice following 1 hr of i.v. live C. albicans. Scale bar, 200 mm. (B and C) (B) Mean cluster area and (C) max skeletal length of clusters were compared. One-way ANOVA, Holm-Sidak’s multiple comparison. (D and E) Lung LTB4 levels of C57BL/6 and C3/ mice (D), two-way ANOVA, Holm-Sidak’s multiple comparison, and following anti-C5aR mAb-treated mice (E). (F) LTB4 in isolated pulmonary PMN with or without C. albicans infection (1 hr). Unpaired two-tailed t test. (G) A representative image of PMN clusters (white) in LTB4R/ mice following C. albicans (1 hr). Scale bar, 200 mm. (H and I) (H) Mean cluster area and (I) max. skeletal length of PMN clusters after C. albicans (1 hr). (J–L) 1 3 107 A488 zymosan or 1 3 107 A488 E. coli bioparticles (green) were injected i.v. during lung imaging. (J) Intravital images are shown at 60 min. Scale bar, 100 mm. Clusters were quantiﬁed as (K) max skeletal length and (L) mean cluster area. ANOVA was performed for panels with more than two groups, while unpaired two-tailed t test were used to compare two groups. n = at least 3 for each panel. Error bars represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Ly6G (clone 1A8) BioLegend RRID: AB_2563207 (BioLegend Cat. No. 127636) Anti-CD31 (clone MEC13.3) BioLegend RRID: AB_2161030 (BioLegend Cat. No. 102515) Anti-CD45 (clone 30-F11) BioLegend RRID: AB_493532 (BioLegend Cat. No. 103121) Anti-CD49b (clone HMa2) BD Bioscience Catalog No. 558295 Anti-C5aR (CD88, clone 20/70) BioLegend RRID: AB_2067286 (BioLegend Cat. No. 135804) Purified anti-mouse Ly6G (clone 1A8) BioXCell BP0075-1 Anti-TER119 Biolegend RRID: AB_528961 (BioLegend Cat. No. 116218) Anti-human CD15 (clone W6D3) BioLegend RRID: AB_756018 (BioLegend Cat. No. 323012) Anti-human CD45 (clone 2D1) R&D Systems Catalog # FAB1430G Anti-human C5aR (CD88, clone S5/1) BioLegend RRID: AB_2259318 (BioLegend Cat. No. 344302) Bacterial and Virus Strains C. albicans (strain ATCC58716) ATCC LUMC-101 Chemicals, Peptides, and Recombinant Proteins Zymosan A (S. cerevisiae) BioParticles ThermoFisher Z23373 Escherichia coli (K-12 strain) BioParticles ThermoFisher E13231 SYTO 9 Green Fluorescent Nucleic Acid Stain ThermoFisher S34854 Compstatin Tocris Cat. No. 2585 LY293111 Cayman Chemical Company CAS N 161172-51-6 Recombinant mouse C5a R&D Systems 2150-C5-025 Critical Commercial Assays LTB4 Parameter Assay Kit R&D Systems KGE006B EasySep Mouse Biotin Positive Selection Kit STEMCELL Technologies Catalog # 18556 Mouse Complement C5a PicoKine ELISA Kit Boster Biological Technology EK0987 Polydimethylsiloxane (PDMS) microfluidic devices SynvivoBio Cat # 104001 Experimental Models: Cell Lines human lung microvascular endothelial cells HLMEC Lonza CC-2527 Experimental Models: Organisms/Strains C57BL/6J The Jackson Laboratory Stock No: 000664 LTB4R knockout (B6.129S4-Ltb4r1tm1Adl/J) The Jackson Laboratory Stock No: 008102 C3 / ; (B6;129S4-C3tm1Crr/J) Gift from Paul Kubes N/A CD11b / ; (B6.129S4-Itgamtm1Myd/J) Gift from Paul Kubes N/A CD11a / ; (B6.129S7-Itgaltm1Bll/J) Gift from Paul Kubes N/A LysM-GFP Gift from Paul Kubes N/A Ly6G-cre (C57BL/6-Ly6g(tm2621(CretdTomato)Arte)) Gift from Matthias Gunzer N/A Software and Algorithms Volocity PerkinElmer N/A Leica LAS Leica N/A Prism 7 software GraphPad V7.02 FlowJo FlowJo V10.1 e1 Cell Host & Microbe 23, 1–13.e1–e4, January 10, 2018

Techniques: In Vivo, Comparison, Isolation, Infection, Two Tailed Test, Injection, Imaging

Figure 5. LTB4 Is Not Required for Host Defense but Drives PMN Clustering, Pulmonary Hemorrhage, Hypoxemia, and Worse Clinical Outcomes (A) Quantiﬁcation of C. albicans phagocytosed during the initial 10 min of infection. (B) The number of PMN chemotaxing to individual C. albicans. (C) PMN displacement during chemotaxis was quantiﬁed. (D) Fungal CFU in the organs of mice 1 hr and 24 hr after C. albicans infection. Unpaired two-tailed t test. (E) Pulmonary blood ﬂow was visualized using ﬂuorescent dextran (green) i.v. without or 1 hr after C. albicans. Scale bar represents 75 mm. (F) Hypoxemia was determined using pulse oximetry and (G) pulmonary hemorrhage was quantiﬁed in the BAL using ﬂow following 4 hr of intravenous C. albicans. (H) Histology following 4 hr of infection or control. (I) Hypothermia and (G) clinical severity scores were measured 24 hr after C. albicans infection. Unpaired two-tailed t test. For (A)–(C), (E), and (H) represent a minimum of n = 3. For other panels, n = the symbols shown. Error bars represent mean ± SEM. *p < 0.05, **p < 0.01.

Journal: Cell host & microbe

Article Title: Leukotriene B4-Mediated Neutrophil Recruitment Causes Pulmonary Capillaritis during Lethal Fungal Sepsis.

doi: 10.1016/j.chom.2017.11.009

Figure Lengend Snippet: Figure 5. LTB4 Is Not Required for Host Defense but Drives PMN Clustering, Pulmonary Hemorrhage, Hypoxemia, and Worse Clinical Outcomes (A) Quantiﬁcation of C. albicans phagocytosed during the initial 10 min of infection. (B) The number of PMN chemotaxing to individual C. albicans. (C) PMN displacement during chemotaxis was quantiﬁed. (D) Fungal CFU in the organs of mice 1 hr and 24 hr after C. albicans infection. Unpaired two-tailed t test. (E) Pulmonary blood ﬂow was visualized using ﬂuorescent dextran (green) i.v. without or 1 hr after C. albicans. Scale bar represents 75 mm. (F) Hypoxemia was determined using pulse oximetry and (G) pulmonary hemorrhage was quantiﬁed in the BAL using ﬂow following 4 hr of intravenous C. albicans. (H) Histology following 4 hr of infection or control. (I) Hypothermia and (G) clinical severity scores were measured 24 hr after C. albicans infection. Unpaired two-tailed t test. For (A)–(C), (E), and (H) represent a minimum of n = 3. For other panels, n = the symbols shown. Error bars represent mean ± SEM. *p < 0.05, **p < 0.01.

Techniques: Infection, Chemotaxis Assay, Two Tailed Test, Control

Figure 7. Anti-fungal Activity and Clustering of Human PMN Is Complement and LTB4 Dependent (A) Images of C5aR inhibition on PMN and C. albicans interactions in a human lung microvascular chamber (anti-C5aR-antibody 20 mg/mL). (B) C. albicans phagocytosis by PMN. (C) Residual immobilized but non-phagocytosed yeast PMN. (D and E) (D) Cluster formation and (E) the number of clustered PMN. (F) Images of LTB4R inhibition on PMN and C. albicans interactions in a human lung microvascular chamber. (G) Quantiﬁcation of immobilized but non-phagocytosed yeast at 30 min. (H and I) (H) Clusters per ﬁeld of view (FOV) and (I) PMN that were clustered per FOV were determined at 30 min. All data are expressed as mean ± SEM for n = 5–7. Student’s t tests were used to compare data between two groups. *p < 0.05 and **p < 0.01. All scale bars represent 20 mm.

Journal: Cell host & microbe

Article Title: Leukotriene B4-Mediated Neutrophil Recruitment Causes Pulmonary Capillaritis during Lethal Fungal Sepsis.

doi: 10.1016/j.chom.2017.11.009

Figure Lengend Snippet: Figure 7. Anti-fungal Activity and Clustering of Human PMN Is Complement and LTB4 Dependent (A) Images of C5aR inhibition on PMN and C. albicans interactions in a human lung microvascular chamber (anti-C5aR-antibody 20 mg/mL). (B) C. albicans phagocytosis by PMN. (C) Residual immobilized but non-phagocytosed yeast PMN. (D and E) (D) Cluster formation and (E) the number of clustered PMN. (F) Images of LTB4R inhibition on PMN and C. albicans interactions in a human lung microvascular chamber. (G) Quantiﬁcation of immobilized but non-phagocytosed yeast at 30 min. (H and I) (H) Clusters per ﬁeld of view (FOV) and (I) PMN that were clustered per FOV were determined at 30 min. All data are expressed as mean ± SEM for n = 5–7. Student’s t tests were used to compare data between two groups. *p < 0.05 and **p < 0.01. All scale bars represent 20 mm.

Techniques: Activity Assay, Inhibition

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